PURIFICATION, CRYSTALLIZATION, AND PRELIMINARY-X-RAY ANALYSIS OF PEPX, AN X-PROLYL DIPEPTIDYL AMINOPEPTIDASE FROM LACTOCOCCUS-LACTIS

被引:7
作者
CHICH, JF
RIGOLET, P
NARDI, M
GRIPON, JC
RIBADEAUDUMAS, B
BRUNIE, S
机构
[1] Unité de Biochimie et de Structure des Protéines, INRA, Centre de Recherches de Jouy-en-Josas, Jouy-En-Josas
来源
PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1995年 / 23卷 / 02期
关键词
OVERPRODUCTION; CRYSTALLOGRAPHY; X-PROLYL DIPEPTIDYL AMINOPEPTIDASE; PEPX; LACTOCOCCUS LACTIS;
D O I
10.1002/prot.340230216
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The X-prolyl dipeptidyl aminopeptidase PepX, a serine peptidase isolated originally from Lactococcus lactis subsp lactis NCDO 763, was cloned and overproduced in Escherichia coli. The enzyme was isolated in its active form in two purification steps. Crystals of PepX were grown by the hanging drop vapor diffusion method using polyethyleneglycol 4000 as precipitant at pH 5.0. The crystals are orthorhombic with cell dimensions a=92.8 Angstrom, b = 102.6 Angstrom, and c=101.6 Angstrom, space group P2(1)2(1)2, and probably contain one monomer of 87.5 kDa in the asymmetric unit. The crystals, very stable under X-rays, diffract to at least 2.2 Angstrom and are suitable for high-resolution structural analysis. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:278 / 281
页数:4
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