2 PATHOGEN-RESPONSIVE GENES IN PARSLEY ENCODE A TYROSINE-RICH HYDROXYPROLINE-RICH GLYCOPROTEIN (HRGP) AND AN ANIONIC PEROXIDASE

被引：42

作者：

KAWALLECK, P ^{[1
]}

SCHMELZER, E ^{[1
]}

HAHLBROCK, K ^{[1
]}

SOMSSICH, IE ^{[1
]}

机构：

[1] MAX PLANCK INST ZUCHTUNGSFORSCH, BIOCHEM ABT, D-50829 COLOGNE, GERMANY

来源：

MOLECULAR AND GENERAL GENETICS | 1995年 / 247卷 / 04期

关键词：

PETROSELINUM CRISPUM; IN SITU RNA RNA HYBRIDIZATION; PHYTOPHTHORA MEGASPERMA; LOCALIZED PATHOGEN DEFENSE; TRANSCRIPTIONAL REGULATION;

D O I：

10.1007/BF00293146

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two recently isolated cDNAs representing genes that are transcriptionally activated in fungus-infected parsley leaves or elicitor-treated, cultured parsley cells are shown to encode a hydroxyproline-rich glyco-protein (HRGP) and an anionic peroxidase. The deduced HRGP protein is rich in tyrosine residues, a feature also found in other pathogen- and wound-induced plant HRGPs. Expression of the peroxidase gene(s) is induced rapidly upon elicitation and precedes that of the HRGP gene. In situ hybridization experiments demonstrate the presence of HRGP and peroxidase mRNAs in parsley tissue around fungal infection sites. Peroxidase mRNA accumulation is particularly sharply restricted to plant cells directly adjacent to fungal hyphae. These results provide further evidence for an important role of specific cell wall modifications in plant defense.

引用

页码：444 / 452

页数：9