PHASE-RESOLVED FIBEROPTICS FLUOROIMMUNOSENSOR

被引：28

作者：

VO-DINH, T ^{[1
]}

NOLAN, T ^{[1
]}

CHENG, YF ^{[1
]}

SEPANIAK, MJ ^{[1
]}

ALARIE, JP ^{[1
]}

机构：

[1] UNIV TENNESSEE, DEPT CHEM, KNOXVILLE, TN 37916 USA

来源：

APPLIED SPECTROSCOPY | 1990年 / 44卷 / 01期

关键词：

D O I：

10.1366/0003702904085859

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

This paper describes the development and application of a phase-resolved fiber-optics fluoroimmunosensor (PR-FIS), which combines the biochemical specificity of antigen-antibody reaction, the high sensitivity of laser excitation, the versatility of fiber-optics sensors, and the selectivity of phase-resolved detection. The PR-FIS device can differentiate benzo(a)pyrene and benzo(a)pyrene tetrol, a DNA-adduct product of the carcinogen benzo(a)pyrene. Benzo(a)pyrene tetrol can be detected at femtomole levels in the presence of an amount of interfacing benzo(a)pyrene that is 50-fold more concentrated.

引用

页码：128 / 132

页数：5

共 12 条

[1] A NEW FIBER-OPTIC-BASED MULTIFREQUENCY PHASE-MODULATION FLUOROMETER
BRIGHT, FV
[J]. APPLIED SPECTROSCOPY, 1988, 42 (08) : 1531 - 1537
[2] Lakowicz JR., 2006, PRINCIPLES FLUORESCE
[3] PHASE-RESOLVED FLUORESCENCE SPECTROSCOPY
MCGOWN, LB
BRIGHT, FV
[J]. ANALYTICAL CHEMISTRY, 1984, 56 (13) : A400 - &
[4] FIBER-OPTIC SENSORS FOR BIOMEDICAL APPLICATIONS
PETERSON, JI
VUREK, GG
[J]. SCIENCE, 1984, 224 (4645) : 123 - 127
[5] MONOCLONAL-ANTIBODIES TO DNA MODIFIED BY A BENZO[A]PYRENE DIOL EPOXIDE
SANTELLA, RM
LIN, CD
CLEVELAND, WL
WEINSTEIN, IB
[J]. CARCINOGENESIS, 1984, 5 (03) : 373 - 377
[6] SEITZ WR, 1984, ANAL CHEM, V56, pA17
[7] FIBEROPTIC CHEMICAL SENSORS FOR COMPETITIVE-BINDING FLUOROIMMUNOASSAY
TROMBERG, BJ
SEPANIAK, MJ
VO-DINH, T
GRIFFIN, GD
[J]. ANALYTICAL CHEMISTRY, 1987, 59 (08) : 1226 - 1230
[8] DEVELOPMENT OF ANTIBODY-BASED FIBER-OPTIC SENSORS FOR DETECTION OF A BENZO[A]PYRENE METABOLITE
TROMBERG, BJ
SEPANIAK, MJ
ALARIE, JP
VO-DINH, T
SANTELLA, RM
[J]. ANALYTICAL CHEMISTRY, 1988, 60 (18) : 1901 - 1908
[9] ANTIBODY-BASED FIBEROPTICS BIOSENSOR FOR THE CARCINOGEN BENZO(A)PYRENE
VO-DINH, T
TROMBERG, BJ
GRIFFIN, GD
AMBROSE, KR
SEPANIAK, MJ
GARDENHIRE, EM
[J]. APPLIED SPECTROSCOPY, 1987, 41 (05) : 735 - 738
[10] A PORTABLE FIBEROPTIC MONITOR FOR FLUOROMETRIC BIOASSAYS
VO-DINH, T
GRIFFIN, GD
AMBROSE, KR
[J]. APPLIED SPECTROSCOPY, 1986, 40 (05) : 696 - 700

← 1 2 →