DIFFERENTIAL EXPRESSION OF GLUTATHIONE-S-TRANSFERASE (GST) BY ADULT HELIGMOSOMOIDES-POLYGYRUS DURING PRIMARY INFECTION IN FAST AND SLOW RESPONDING HOSTS

被引：16

作者：

BROPHY, PM ^{[1
]}

BENSMITH, A ^{[1
]}

BROWN, A ^{[1
]}

BEHNKE, JM ^{[1
]}

PRITCHARD, DI ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,DEPT LIFE SCI,NOTTINGHAM NG2 7RD,ENGLAND

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1995年 / 25卷 / 05期

基金：

英国惠康基金;

关键词：

GLUTATHIONE S-TRANSFERASE; NEMATODE; LIPID PEROXIDATION; VACCINATION; HELIGMOSOMOIDES POLYGYRUS;

D O I：

10.1016/0020-7519(94)00151-D

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Glutathione S-transferase (GST) specific enzymatic activity, assayed with the model substrate 1-chloro-2,4-dinitrobenzene, was 45% higher in adult Heligmosomoides polygyrus passaged through a slow responder mouse strain, C57/BL10 compared to worms passaged through a fast-responder strain (SWR X SJL) F-1. Western analysis using polyclonal antisera raised to purified H. polygyrus GSTs did not appear to positively correlate the expression of GST protein with functional enzymatic activity. However, western blotting did indicate a sex-linked expression pattern of GST protein, with male worms expressing a higher ratio of the 24 kDa to the 23 kDa GST family than female worms.

引用

页码：641 / 645

页数：5

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