SACCHAROMYCES-CEREVISIAE CELLS SECRETING AN ASPERGILLUS-NIGER BETA-GALACTOSIDASE GROW ON WHEY PERMEATE

被引：71

作者：

KUMAR, V

RAMAKRISHNAN, S

TEERI, TT

KNOWLES, JKC

HARTLEY, BS

机构：

[1] Centre For Biotechnology, Imperial College of Science and Technology, London

[2] V.T.T Biotechnical Laboratory, Espoo, SF-02151

[3] Glaxo Institute of Molecular Biology, Geneva, Plan-les-Ouates, 1228, 14 Chemin des Anlx

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 01期

关键词：

D O I：

10.1038/nbt0192-82

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermo-stable beta-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total beta-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

引用

页码：82 / 85

页数：4

共 30 条

[1]

AMMERER G, 1983, METHOD ENZYMOL, V101, P192

[2] TY ELEMENTS TRANSPOSE THROUGH AN RNA INTERMEDIATE [J].