EFFECT OF CALCIUM-IONS ON THE PROCESSING OF PROOPIOMELANOCORTIN BY BOVINE INTERMEDIATE LOBE PRO-OPIOMELANOCORTIN-CONVERTING ENZYME

被引:8
作者
BIRCH, NP
BENNETT, HPJ
ESTIVARIZ, FE
LOH, YP
机构
[1] NICHHD,DEV NEUROBIOL LAB,CELLULAR NEUROBIOL SECT,BLDG 36,ROOM 2A-21,9000 ROCKVILLE PIKE,BETHESDA,MD 20892
[2] ROYAL VICTORIA HOSP,MONTREAL H3A 1A1,QUEBEC,CANADA
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 201卷 / 01期
关键词
D O I
10.1111/j.1432-1033.1991.tb16259.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of Ca2+ on the extent and pattern of processing of pro-opiomelanocortin and an N-terminal fragment by a purified pituitary secretory vesicle, soluble aspartic endoprotease, was studied. Ca2+ stimulated the first cleavage of pro-opiomelanocortin by pro-opiomelanocortin-converting enzyme to yield 21 - 23 kDa adrenocorticotropin and beta-lipotropin, but its effect was minimal. The production of adrenocorticotropin from the 21 - 23 kDa intermediate was stimulated approximately 2.3-fold in the presence of 10 mM Ca2+, and processing of beta-lipotropin to beta-endorphin was stimulated about 1.3 - 1.4-fold by 5-10 mM Ca2+. The production of gamma-melanotropin-immunoreactive material from bovine N-pro-opiomelanocortin(1 - 77) was stimulated approximately 1.3-fold at both 100-mu-M and 1.5-2.0 mM Ca2+. Further characterization of the gamma-melanotropin-immunoreactive material by HPLC demonstrated that the major products were gamma-3-[Lys]melanotropin and gamma-3-melanotropin at both Ca2+ concentrations. These results indicate that pro-opiomelanocortin-converting enzyme is stimulated by Ca2+.
引用
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页码:85 / 89
页数:5
相关论文
共 24 条
[11]   LOW-MOLECULAR-WEIGHT CONSTITUENTS OF ISOLATED INSULIN-SECRETORY GRANULES - BIVALENT-CATIONS, ADENINE-NUCLEOTIDES AND INORGANIC-PHOSPHATE [J].
HUTTON, JC ;
PENN, EJ ;
PESHAVARIA, M .
BIOCHEMICAL JOURNAL, 1983, 210 (02) :297-305
[12]   USE OF REVERSED-PHASE AND ION-EXCHANGE BATCH EXTRACTION IN THE PURIFICATION OF BOVINE PITUITARY PEPTIDES [J].
JAMES, S ;
BENNETT, HPJ .
JOURNAL OF CHROMATOGRAPHY, 1985, 326 (JUN) :329-338
[13]   INFLUX OF CA-2+ INTO ISOLATED SECRETORY VESICLES FROM ADRENAL-MEDULLA - INFLUENCE OF EXTERNAL K+ AND NA+ [J].
KRIEGERBRAUER, H ;
GRATZL, M .
FEBS LETTERS, 1981, 133 (02) :244-246
[14]  
LEMOS JR, 1989, SECRETION ITS CONTRO, P333
[15]   PROTEOLYSIS IN NEUROPEPTIDE PROCESSING AND OTHER NEURAL FUNCTIONS [J].
LOH, YP ;
BROWNSTEIN, MJ ;
GAINER, H .
ANNUAL REVIEW OF NEUROSCIENCE, 1984, 7 :189-222
[16]  
LOH YP, 1986, J BIOL CHEM, V261, P1949
[17]  
LOH YP, 1984, J BIOL CHEM, V259, P8238
[18]  
LOH YP, 1985, J BIOL CHEM, V260, P7194
[19]   DIRECT EFFECTS OF GLUCOSE ON PROINSULIN SYNTHESIS AND PROCESSING DURING DESENSITIZATION [J].
NAGAMATSU, S ;
BOLAFFI, JL ;
GRODSKY, GM .
ENDOCRINOLOGY, 1987, 120 (04) :1225-1231
[20]  
PARISH DC, 1986, J BIOL CHEM, V261, P4392