INFLUENCE OF AMINO-ACIDS ON THE FORMATION OF MUTAGENIC/CARCINOGENIC HETEROCYCLIC AMINES IN A MODEL SYSTEM

被引:74
作者
JOHANSSON, MAE
FAY, LB
GROSS, GA
OLSSON, K
JAGERSTAD, M
机构
[1] NESTEC LTD,NESTLE RES CTR,CH-1000 LAUSANNE 26,SWITZERLAND
[2] SWEDISH UNIV AGR SCI,DEPT CHEM,S-75007 UPPSALA,SWEDEN
关键词
D O I
10.1093/carcin/16.10.2553
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Mixtures of creatinine, glucose and various single amino acids were heated at 180 degrees C for 10 min in an aqueous model system. The heated mixtures all showed mutagenic activity, ranging from 80 to 2400 TA98 revertant colonies/mu mol creatinine with metabolic activation, Testing of HPLC fractions for mutagenic activity showed each mixture to contain several mutagenic components, some of which corresponded to known heterocyclic amines and others to unknown compounds, The presence of 2-amino-3-methyl-imidazo[4,5-f]quinoxaline, 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline and 2-amino-3,7,8-trimethylimidazo[4,5-f]quinoxaline in most of the samples was established using HPLC with photodiode array detection and liquid chromatography/mass spectrometry with electrospray interface and single ion monitoring, In addition, 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole and 3-amino-1-methyl-5H-pyrido[4,3-b]indole and the co-mutagenic compounds 9H-pyrido[3,4-b]indole and 1-methyl-9H-pyrido[3,4-b]indole were detected in some samples.
引用
收藏
页码:2553 / 2560
页数:8
相关论文
共 46 条