CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDY OF A RECOMBINANT CUTINASE FROM FUSARIUM-SOLANI-PISI

被引：18

作者：

ABERGEL, C

MARTINEZ, C

FONTECILLACAMPS, J

CAMBILLAU, C

DEGEUS, P

LAUWEREYS, M

机构：

[1] FAC MED NORD,CRISTALLOG & CRISTALLISAT MACROMOLEC BIOL LAB,CNRS,URA 1296,BD P DRAMARD,F-13326 MARSEILLE 15,FRANCE

[2] PLANT GENET SYST NV,B-9000 GHENT,BELGIUM

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 215卷 / 02期

关键词：

D O I：

10.1016/S0022-2836(05)80339-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant cutinase from Fusarium solani pisi is expressed and excreted with very high yields in Escherichia coli cultures. Cutinase was crystallized at 20°C using the vapour diffusion technique, with polyethylene glycol 6000 as precipitant. Best crystals were obtained at pH 7·0 with polyethylene glycol at 15 to 20%. They are monoclinic, with space group P21 and cell dimensions a = 35·1 Å, b = 67·4 Å, c = 37·05 Å and β = 94·0°; they diffract beyond 1·5 Å resolution. The asymmetric unit contains one molecule of 22,000 Da (Vm = 1·98 Å3/Da; 38% water). © 1990 Academic Press Limited.

引用

页码：215 / 216

页数：2

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