SOLUBLE GLYCOPROTEINS FROM SUGAR-CANE JUICE ANALYZED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND FLUORESCENCE EMISSION

被引：21

作者：

LEGAZ, ME ^{[1
]}

PEDROSA, MM ^{[1
]}

DEARMAS, R ^{[1
]}

MARTINEZ, MM ^{[1
]}

VICENTE, C ^{[1
]}

机构：

[1] UNIV HAVANA,FAC BIOL,DEPT PLANT PHYSIOL,HAVANA,CUBA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 697卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)00948-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Soluble polysaccharides have been isolated from sugar cane by size-exclusion chromatography using two successive columns of-Sephadex-G-10 and G-50. Two main fractions of different molecular mass were separated and their homogeneity was tested by HPLC using a Zorbax GF-450 and a Zorbax GF-250 column in series. Whereas the high-molecular-mass soluble polysaccharide (SP) fraction seems to be composed of two different polymers with retention times of about 16.5 and 24.5 min, the mid-molecular-mass carbohydrate (MMMC) fraction is resolved as only one peak with a retention time of about 2.45 min. This implies that Sephadex G-50 is not able to discriminate between the two categories of macromolecules, as SP eluted from Sephadex G-50 is clearly contaminated by MMMC. Fractionation of clarified juice with 80% (v/v) ethanol separates SP as an insoluble fraction and MMMC as an ethanol-soluble macromolecule. The natural fluorescence of both polymers reveals that they are glycoproteins and, in addition, they can clearly be recognized by the degree of tryptophan exposure to its chemical microenvironment.

引用

页码：329 / 335

页数：7

共 19 条

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