PROLIFERATIVE ASSESSMENT OF GFAP-POSITIVE AND GFAP-NEGATIVE GLIOMA-CELLS BY NUCLEOLAR ORGANIZER REGION STAINING

被引：15

作者：

HARA, A ^{[1
]}

SAKAI, N ^{[1
]}

YAMADA, H ^{[1
]}

NIIKAWA, S ^{[1
]}

OHNO, T ^{[1
]}

TANAKA, T ^{[1
]}

MORI, H ^{[1
]}

机构：

[1] GIFU UNIV,SCH MED,DEPT PATHOL,GIFU 500,JAPAN

来源：

SURGICAL NEUROLOGY | 1991年 / 36卷 / 03期

关键词：

NUCLEOLAR ORGANIZER REGIONS; PROLIFERATIVE POTENTIAL; HIGH-GRADE GLIOMA; GLIAL FIBRILLARY ACIDIC PROTEIN;

D O I：

10.1016/0090-3019(91)90111-L

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

The proliferative potential of two types of tumor cells that did or did not express glial fibrillary acidic protein (GFAP) in 19 cases of high-grade gliomas, including nine anaplastic astrocytomas (WHO criteria, grade 3) and 10 glioblastoma multiforme (grade 4), was investigated by a combined staining technique, one-step silver colloid method for nucleolar organizer region-associated argyrophilic protein (Ag-NOR) and immunocytochemistry for GFAP. The mean numbers of Ag-NORs in GFAP-positive cells and GFAP-negative cells of high-grade gliomas were 2.68 and 3.74, respectively. The value of GFAP-negative cells was significantly greater than that of GFAP-positive cells (P < 0.01). Our results show that, by means of the immunocytochemistry for GFAP, tumor cells in human high-grade gliomas can be divided into two groups expressing GFAP or not, and that the mean number of Ag-NORs of GFAP-negative cells is more representative of the degree of histological malignancy than that of positive cells. It is considered that the proliferative assessment of GFAP-negative tumor cells in high-grade gliomas by combined staining of Ag-NOR silver staining and GFAP immunocytochemistry is useful for understanding the histological malignancy of high-grade gliomas.

引用

页码：190 / 194

页数：5

共 19 条

[1] NUCLEOLAR ORGANIZER REGIONS IN SMALL CELL-CARCINOMA OF THE BRONCHUS [J].