CONDUCTIMETRIC ASSAY OF PYROGLUTAMYL PEPTIDASE ACTIVITY

被引：2

作者：

BESSON, C

VESSILLIER, S

GONZALES, T

SAULNIER, J

WALLACH, J

机构：

[1] ICBMC, BIOCHIM ANALYT & SYNTH BIOORGAN LAB, F-69622 VILLEURBANNE, FRANCE

[2] INST NATL SCI APPL, GENET MOLEC MICROORGANISMES & INTERACT CELLULAIRE, CNRS, URA 1486, F-69621 VILLEURBANNE, FRANCE

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 294卷 / 03期

关键词：

CONDUCTIMETRY; SPECTROPHOTOMETRY; PYROGLUTAMYL PEPTIDASE;

D O I：

10.1016/0003-2670(94)80314-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A conductimetric method was applied to assay Streptococcus pyogenes pyroglutamyl peptidase (E.C. 3.4.8.11) activity using pyroglutamylalanine or pyroglutamyltyrosine. A spectrophotometric comparison using pyroglutamyl-beta-naphthylamide indicated that the sensitivity was much increased when using conductimetry. The use of a C-protected dipeptide such as pyroglutamylalanineamide also increased the sensitivity. Kinetic parameters have been measured for both dipeptides, with K-M values of 0.34 mM and 0.47 mM, respectively, for the alanine and tyrosine derivatives.

引用

页码：305 / 309

页数：5

共 15 条

[1] ONE-STEP PURIFICATION AND CHARACTERIZATION OF THE PYRROLIDONE CARBOXYL PEPTIDASE OF STREPTOCOCCUS-PYOGENES OVER-EXPRESSED IN ESCHERICHIA-COLI [J].