ANALYSIS OF RNA SECONDARY STRUCTURE BY PHOTO-CHEMICAL REVERSAL OF PSORALEN CROSSLINKS

被引：49

作者：

RABIN, D

CROTHERS, DM

机构：

[1] Department of Chemistry, Yale University, New Haven

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/7.3.689

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aminomethyltrioxsalen (AMT), a psoralen, is known to cause interstrand crosslinks in double stranded nucleic acids. We have demonstrated the photochemical reversal of this reaction, and have used this result to develop a method for identification of specific sequences which are adjacent because of RNA secondary structure formation. E. coli 5S rRNA is used as a model system. We isolated and characterized a product that is derived from the stem region of 5S RNA. © 1979 Information Retrieval Limited.

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页码：689 / 703

页数：15

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