AXENIC CULTURE AND ENCAPSULATION OF THE INTRARADICAL FORMS OF GLOMUS SPP

被引：15

作者：

STRULLU, DG

ROMAND, C

PLENCHETTE, C

机构：

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1991年 / 7卷 / 03期

关键词：

D O I：

10.1007/BF00329394

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In recent years there have been many attempts to cultivate in vitro vesicular-arbuscular mycorrhizal (VAM) fungi which are obligate symbionts. Resting spores extracted from soils are often used as inoculum. Mycorrhizal root pieces are also used for inoculation but the role of intra-radical structures has not been clearly established. On agar medium vegetative mycelium was regenerated from individual intra-radical vesicles and from hyphae extracted by enzymatic maceration. After cell penetration, the mycelium probably accumulates substances which allow growth of VAM fungi in pure culture. When associated with tomato roots, this mycelium forms typical mycorrhizae. Encapsulation stabilized the biological properties of mycorrhizal roots and isolated vesicles. The immobilization also preserved the infectivity of the intra-radical hyphae and vesicles. After 25 years of exclusive utilization of resting spores as starting material for axenic and dual cultures of VAM fungi, it appears that intra-radical vesicles may be preferable propagules.

引用

页码：292 / 297

页数：6

共 30 条

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