MINIMAL REQUIREMENTS FOR EXPONENTIAL-GROWTH OF LACTOCOCCUS-LACTIS

被引：274

作者：

JENSEN, PR

HAMMER, K

机构：

[1] TECH UNIV DENMARK,DEPT MICROBIOL,DK-2800 LYNGBY,DENMARK

[2] NETHERLANDS CANC INST H5,DIV MOLEC BIOL,1066 CX AMSTERDAM,NETHERLANDS

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 12期

关键词：

D O I：

10.1128/AEM.59.12.4363-4366.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of mu = 0.3 h-1. With 19 amino acids added, the growth rate increased to mu = 0.7 h-1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.

引用

页码：4363 / 4366

页数：4