IDENTIFICATION OF IN-VIVO PHOSPHORYLATION SITES OF SET, A NUCLEAR PHOSPHOPROTEIN ENCODED BY THE TRANSLOCATION BREAKPOINT IN ACUTE UNDIFFERENTIATED LEUKEMIA

被引：43

作者：

ADACHI, Y ^{[1
]}

PAVLAKIS, GN ^{[1
]}

COPELAND, TD ^{[1
]}

机构：

[1] NCI, FREDERICK CANC RES & DEV CTR, ABL BASIC RES PROGRAM, HUMAN RETROVIRUS SECT, FREDERICK, MD 21702 USA

来源：

FEBS LETTERS | 1994年 / 340卷 / 03期

关键词：

SET; NUCLEAR PROTEIN; PROTEIN PHOSPHORYLATION; TRANSLOCATION; ACUTE UNDIFFERENTIATED LEUKEMIA;

D O I：

10.1016/0014-5793(94)80144-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SET, the translocation breakpoint-encoded protein in acute undifferentiated leukemia (AUL), is identified as a 39-kDa phosphoprotein found predominantly in the cell nuclei [1994, J. Biol. Chem. 269, 2258-2262]. SET is fused to a putative oncoprotein, CAN, in AUL and is thought to regulate the transformation potential of SET-CAN by its nuclear localization and phosphorylation. We investigated in detail the in vivo phosphorylation of SET. Phosphorylation of SET occurred in all human cell lines examined in vivo, primarily on serine residues. Endoproteinase Glu-C digestion of phosphorylated SET yielded two phosphopeptides. By radiosequencing, we identified the in vivo phosphorylation sites of SET as Ser(9) and Se-24. The surrounding sequences of Se-9 and Ser(24) contained an apparent consensus site sequence for protein kinase C.

引用

页码：231 / 235

页数：5

共 28 条

[1] EFFICIENT LARGE-SCALE PURIFICATION OF NONHISTONE CHROMOSOMAL-PROTEINS HMG1 AND HMG2 BY USING POLYBUFFER-EXCHANGER PBE94 [J].