MOLECULAR-CLONING AND CHARACTERIZATION OF AN IMMUNOSUPPRESSIVE AND WEAKLY ONCOGENIC VARIANT OF FRIEND MURINE LEUKEMIA-VIRUS, FIS-2

被引:9
作者
DAI, HY
FAXVAAG, A
TROSETH, GI
AARSET, H
DALEN, A
机构
[1] UNIV TRONDHEIM, INST CANC RES, N-7005 TRONDHEIM, NORWAY
[2] TRONDHEIM REG & UNIV HOSP, DEPT PATHOL, TRONDHEIM, NORWAY
[3] TRONDHEIM REG & UNIV HOSP, DEPT MICROBIOL, TRONDHEIM, NORWAY
关键词
D O I
10.1128/JVI.68.11.6976-6984.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The FIS variant is weakly leukemogenic, relatively strong immunosuppressive murine retrovirus which was isolated from the T helper cells of adult NMRI mice infected with Friend murine leukemia virus (F-MuLV) complex (FV). Unlike FV, it does not induce acute erythroleukemia but retains the immunosuppressive property of FV and induces suppression of the primary antibody response rapidly and persistently in adult mice. A previous study showed that the FIS variant contains two viral components, a replication-competent virus and a defective virus. In this study, we have biologically purified the FIS variant by end point dilution and we show that the replication-competent virus FIS-2 along can induce immunosuppression as the parental FIS variant. Most newborn mice infected with FIS-2 developed erythroleukemia, but with an increased latency period compared with that of F-MuLV clone 57. In contrast, FIS-S-2 induced suppression of the primary antibody response and disease more rapidly than F-MuLV clone 57 in immunocompetent, adult mice. FIS-2 was further molecularly cloned and characterized. Restriction mapping and nucleotide sequence analysis of FIS-2 showed a high degree of homology between FIS-2 and F-MuLV clone 57; suggesting that FIS-E is a variant of F-MuLV. The striking difference is the deletion of one of the tandem repeats in the FIS-2 long terminal repeat and the single point mutation in the binding-sites for core-binding protein and FVa compared with the long terminal repeat of F-MuLV clone 57. Two single point mutations led to the appearance of two extra potential N glycosylation sites in the FIS-2 gag-encoded glycoprotein. Together, the results suggest that FIS-2 represents an interesting murine model to study retrovirus-induced immunosuppression on the basis of its unique combined property of low leukemogenicity and relatively strong and persistent immunosuppressive activity adult mice.
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页码:6976 / 6984
页数:9
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