APPLICATIONS OF AUTOMATED AMINO-ACID-ANALYSIS USING 9-FLUORENYLMETHYL CHLOROFORMATE

被引：76

作者：

HAYNES, PA ^{[1
]}

SHEUMACK, D ^{[1
]}

GREIG, LG ^{[1
]}

KIBBY, J ^{[1
]}

REDMOND, JW ^{[1
]}

机构：

[1] ICI INSTRUMENTS,DINGLEY,VIC 3172,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 588卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(91)85012-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A rapid and sensitive fully automated method for the determination of primary and secondary amino acids in different matrices is described. Amino acids are derivatized with 9-fluorenylmethyl chloroformate using an automated precolumn derivatization technique. Data are presented to show that the technique is both reproducible and highly sensitive. Applications of the technique are presented, including the analysis of peptide and protein hydrolysates and the profiling of free amino acids in physiological fluids.

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页码：107 / 114

页数：8

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