4,5-DIAMINOPHTHALHYDRAZIDE AS A HIGHLY SENSITIVE CHEMILUMINESCENCE DERIVATIZATION REAGENT FOR ALPHA-DICARBONYL COMPOUNDS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：17

作者：

ISHIDA, J ^{[1
]}

SONEZAKI, S ^{[1
]}

YAMAGUCHI, M ^{[1
]}

机构：

[1] FUKUOKA UNIV,FAC PHARMACEUT SCI,JOHNAN KU,FUKUOKA 81401,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 598卷 / 02期

关键词：

D O I：

10.1016/0021-9673(92)85049-Y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

4,5-Diaminophthalhydrazide (DPH) was found to be a highly sensitive chemiluminescence derivatization reagent for alpha-dicarbonyl compounds in high-performance liquid chromatography. The reagent reacts with alpha-dicarbonyl compounds in dilute hydrochloric acid in the presence of beta-mercaptoethanol to give highly chemiluminescent quinoxaline derivatives which produce chemiluminescence by reaction with hydrogen peroxide and potassium hexacyanoferrate(III). The DPH derivatives of five alpha-dicarbonyl compounds were separated on a reversed-phase column, TSKgel ODS-120T, with a mixture of acetonitrile and 10 mM ammonium acetate, followed by chemiluminescence detection. The detection limits are in the range 1.1-300 fmol for a 20-mu-l injection volume (signal-to-noise ratio 3).

引用

页码：203 / 208

页数：6

共 24 条

[1] DETERMINATION OF BIACETYL IN BEER BY LIQUID-CHROMATOGRAPHY WITH SENSITIZED PHOSPHORESCENCE DETECTION [J].

BAUMANN, RA ;

GOOIJER, C ;

VELTHORST, NH ;

FREI, RW ;

STRATING, J ;

VERHAGEN, LC ;

VELDHUYZENDOORDUIN, RC .

INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 1986, 25 (1-3) :195-207

[2] QUANTITATIVE-DETERMINATION OF BLOOD-GLUCOSE USING ENZYME INDUCED CHEMILUMINESCENCE OF LUMINOL [J].

BOSTICK, DT ;

HERCULES, DM .

ANALYTICAL CHEMISTRY, 1975, 47 (03) :447-452

[3]

DAMIANI P, 1988, J ASSOC OFF ANA CHEM, V71, P462

[4] SPECTROPHOTOMETRIC DETERMINATION OF METHYL GLYOXAL WITH 2,4-DINITROPHENYLHYDRAZINE [J].

GILBERT, RP ;

BRANDT, RB .

ANALYTICAL CHEMISTRY, 1975, 47 (14) :2418-2422

[5] 1,2-DIAMINO-4,5-METHYLENEDIOXYBENZENE AS A HIGHLY SENSITIVE FLUOROGENIC REAGENT FOR ALPHA-DICARBONYL COMPOUNDS [J].

HARA, S ;

YAMAGUCHI, M ;

TAKEMORI, Y ;

YOSHITAKE, T ;

NAKAMURA, M .

ANALYTICA CHIMICA ACTA, 1988, 215 (1-2) :267-276

[6] 4,5-DIAMINOPHTHALHYDRAZIDE AS A HIGHLY SENSITIVE CHEMILUMINESCENCE REAGENT FOR ALPHA-KETO ACIDS IN LIQUID-CHROMATOGRAPHY [J].

ISHIDA, J ;

YAMAGUCHI, M ;

NAKAHARA, T ;

NAKAMURA, M .

ANALYTICA CHIMICA ACTA, 1990, 231 (01) :1-6

[7]

ISHIDA J, IN PRESS BIOMED CHRO

[8]

JOHNSON P, 1962, ANAL CHEM, V34, P1389

[9] CHEMI-LUMINESCENCE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING N-(4-AMINOBUTYL)-N-ETHYLISOLUMINOL AS A PRECOLUMN LABELING REAGENT [J].

KAWASAKI, T ;

MAEDA, M ;

TSUJI, A .

JOURNAL OF CHROMATOGRAPHY, 1985, 328 (JUN) :121-126

[10] DETERMINATION OF FLUORESCENT COMPOUNDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CHEMI-LUMINESCENCE DETECTION [J].

KOBAYASHI, S ;

IMAI, K .

ANALYTICAL CHEMISTRY, 1980, 52 (03) :424-427

← 1 2 3 →