4,5-DIAMINOPHTHALHYDRAZIDE AS A HIGHLY SENSITIVE CHEMILUMINESCENCE DERIVATIZATION REAGENT FOR ALPHA-DICARBONYL COMPOUNDS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：17

作者：

ISHIDA, J ^{[1
]}

SONEZAKI, S ^{[1
]}

YAMAGUCHI, M ^{[1
]}

机构：

[1] FUKUOKA UNIV,FAC PHARMACEUT SCI,JOHNAN KU,FUKUOKA 81401,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 598卷 / 02期

关键词：

D O I：

10.1016/0021-9673(92)85049-Y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

4,5-Diaminophthalhydrazide (DPH) was found to be a highly sensitive chemiluminescence derivatization reagent for alpha-dicarbonyl compounds in high-performance liquid chromatography. The reagent reacts with alpha-dicarbonyl compounds in dilute hydrochloric acid in the presence of beta-mercaptoethanol to give highly chemiluminescent quinoxaline derivatives which produce chemiluminescence by reaction with hydrogen peroxide and potassium hexacyanoferrate(III). The DPH derivatives of five alpha-dicarbonyl compounds were separated on a reversed-phase column, TSKgel ODS-120T, with a mixture of acetonitrile and 10 mM ammonium acetate, followed by chemiluminescence detection. The detection limits are in the range 1.1-300 fmol for a 20-mu-l injection volume (signal-to-noise ratio 3).

引用

页码：203 / 208

页数：6

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