DNA-DEPENDENT AND RNA-DEPENDENT DNA-POLYMERASES

被引：203

作者：

STEITZ, TA ^{[1
]}

机构：

[1] YALE UNIV, NEW HAVEN, CT 06520 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1993年 / 3卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0959-440X(93)90198-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A catalytic subdomain of HIV reverse transcriptase has the same structure as that of E. coli Klenow fragment, including three conserved carboxylates whose mutation destroys activity. The structures of Klenow fragment complexed with DNA and with divalent metal ions, taken together with mutagenic studies, are consistent with a two-metal-ion mechanism of phosphoryl transfer at both active sites. The source of processivity in DNA polymerase III is easily understood in terms of the donut shape of its beta-subunit.

引用

收藏

页码：31 / 38

页数：8

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