DNA-DEPENDENT AND RNA-DEPENDENT DNA-POLYMERASES

被引:203
作者
STEITZ, TA [1 ]
机构
[1] YALE UNIV, NEW HAVEN, CT 06520 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0959-440X(93)90198-T
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A catalytic subdomain of HIV reverse transcriptase has the same structure as that of E. coli Klenow fragment, including three conserved carboxylates whose mutation destroys activity. The structures of Klenow fragment complexed with DNA and with divalent metal ions, taken together with mutagenic studies, are consistent with a two-metal-ion mechanism of phosphoryl transfer at both active sites. The source of processivity in DNA polymerase III is easily understood in terms of the donut shape of its beta-subunit.
引用
收藏
页码:31 / 38
页数:8
相关论文
共 33 条
[11]   GENETIC-ANALYSIS OF THE INTERACTION BETWEEN BACTERIOPHAGE-T7 DNA-POLYMERASE AND ESCHERICHIA-COLI THIOREDOXIN [J].
HIMAWAN, JS ;
RICHARDSON, CC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) :9774-9778
[12]   REVERSE-TRANSCRIPTASE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 - FUNCTIONALITY OF SUBUNITS OF THE HETERODIMER IN DNA-SYNTHESIS [J].
HOSTOMSKY, Z ;
HOSTOMSKA, Z ;
FU, TB ;
TAYLOR, J .
JOURNAL OF VIROLOGY, 1992, 66 (05) :3179-3182
[13]  
HUBER HE, 1987, J BIOL CHEM, V262, P16224
[14]   CRYSTAL-STRUCTURE AT 3.5 ANGSTROM RESOLUTION OF HIV-1 REVERSE-TRANSCRIPTASE COMPLEXED WITH AN INHIBITOR [J].
KOHLSTAEDT, LA ;
WANG, J ;
FRIEDMAN, JM ;
RICE, PA ;
STEITZ, TA .
SCIENCE, 1992, 256 (5065) :1783-1790
[15]   3-DIMENSIONAL STRUCTURE OF THE BETA-SUBUNIT OF ESCHERICHIA-COLI DNA POLYMERASE-III HOLOENZYME - A SLIDING DNA CLAMP [J].
KONG, XP ;
ONRUST, R ;
ODONNELL, M ;
KURIYAN, J .
CELL, 1992, 69 (03) :425-437
[16]   INFECTIOUS POTENTIAL OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 REVERSE-TRANSCRIPTASE MUTANTS WITH ALTERED INHIBITOR SENSITIVITY [J].
LARDER, BA ;
KEMP, SD ;
PURIFOY, DJM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (13) :4803-4807
[17]   SITE-SPECIFIC MUTAGENESIS OF AIDS VIRUS REVERSE-TRANSCRIPTASE [J].
LARDER, BA ;
PURIFOY, DJM ;
POWELL, KL ;
DARBY, G .
NATURE, 1987, 327 (6124) :716-717
[18]   SUBUNIT-SELECTIVE MUTAGENESIS INDICATES MINIMAL POLYMERASE-ACTIVITY IN HETERODIMER-ASSOCIATED P51 HIV-1 REVERSE-TRANSCRIPTASE [J].
LEGRICE, SFJ ;
NAAS, T ;
WOHLGENSINGER, B ;
SCHATZ, O .
EMBO JOURNAL, 1991, 10 (12) :3905-3911
[19]   STRUCTURAL CHARACTERIZATION OF REVERSE-TRANSCRIPTASE AND ENDONUCLEASE POLYPEPTIDES OF THE ACQUIRED-IMMUNODEFICIENCY-SYNDROME RETROVIRUS [J].
LIGHTFOOTE, MM ;
COLIGAN, JE ;
FOLKS, TM ;
FAUCI, AS ;
MARTIN, MA ;
VENKATESAN, S .
JOURNAL OF VIROLOGY, 1986, 60 (02) :771-775