AGAROSE-GEL ELECTROPHORESIS OF DENATURED RNA WITH SILVER STAINING

被引：12

作者：

SKOPP, RN ^{[1
]}

LANE, LC ^{[1
]}

机构：

[1] UNIV NEBRASKA, DEPT PLANT PATHOL, LINCOLN, NE 68583 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1988年 / 169卷 / 01期

关键词：

D O I：

10.1016/0003-2697(88)90263-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal- or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert, low conductivity buffer. Following electrophoresis, the gel is stained with a sensitive silver stain. The method produces sharp, well-resolved bands and yields accurate RNA size estimates. Because of its sensitivity and simplicity, it is suitable for routine laboratory use.

引用

页码：132 / 137

页数：6

共 23 条

[21] MOLECULAR-WEIGHT DETERMINATION OF NUCLEIC-ACIDS BY GEL-ELECTROPHORESIS IN NON-AQUEOUS SOLUTION [J].

STAYNOV, DZ ;

PINDER, JC ;

GRATZER, WB .

NATURE-NEW BIOLOGY, 1972, 235 (56) :108-&

[22] SECONDARY STRUCTURE MAPS OF RNA - PROCESSING OF HELA RIBOSOMAL-RNA [J].

WELLAUER, PK ;

DAWID, IB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (10) :2827-2831

[23] A SENSITIVE SILVER STAIN FOR PROTEINS IN AGAROSE GELS [J].

WILLOUGHBY, EW ;

LAMBERT, A .

ANALYTICAL BIOCHEMISTRY, 1983, 130 (02) :353-358

← 1 2 3 →