HORIZONTAL POLYACRYLAMIDE-GEL ELECTROPHORESIS FOR THE SEPARATION OF DNA FRAGMENTS

被引：37

作者：

HAAS, H ^{[1
]}

BUDOWLE, B ^{[1
]}

WEILER, G ^{[1
]}

机构：

[1] FED BUR INVEST ACAD,CTR FORENS SCI RES & TRAINING,QUANTICO,VA 22135

来源：

ELECTROPHORESIS | 1994年 / 15卷 / 02期

关键词：

D O I：

10.1002/elps.1150150126

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A discontinuous borate/formate buffer system is presented for horizontal polyacrylamide gel electrophoresis of DNA fragments. The resolution potential of the system could be altered by changing the total monomer concentration (5-9%T), the concentration of the crosslinker piperazine diacrylamide (2-5%C-PDA), as well as the concentration of formate in the gel (40-120 mM), the leading ion of the buffer system. The separation of DNA fragments would be improved by increasing the migration distance from 22 to 28 cm. This discontinuous polyacrylamide gel electrophoresis system proved highly reproducible.

引用

页码：153 / 158

页数：6