IONIC DEPENDENCE OF ADENOSINE UPTAKE INTO CULTURED ASTROCYTES

被引:7
作者
BENDER, AS
WOODBURY, DM
WHITE, HS
机构
[1] UNIV UTAH,COLL PHARM,DEPT PHARMACOL & TOXICOL,SALT LAKE CITY,UT 84112
[2] UNIV UTAH,SCH MED,DEPT PHYSIOL,DIV NEUROPHARMACOL & EPILEPTOL,SALT LAKE CITY,UT 84112
关键词
ADENOSINE UPTAKE; ASTROCYTE; BRL; 34915; IONOPHORE; ION; OMEPRAZOLE;
D O I
10.1016/0006-8993(94)91173-8
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Adenosine uptake in cultured astrocytes is dependent on various ions and energy metabolism. The Na+-gradient plays an important role, since nigericin, ouabain, amiloride and substitution of Na+ with choline inhibited adenosine uptake. The proton-gradient was of importance, since carbonylcyanide m-chlorophenylhydrozone (CCCP) and omeprazole also inhibited adenosine uptake. Furthermore, adenosine uptake was dependent on Cl- anion. Substitution of Cl- with isethionate, as well as DIDS or furosemide inhibited adenosine uptake. Adenosine uptake was also sensitive to Ca2+ gradient, removal of extracellular Ca2+ and calcimycin inhibited adenosine uptake. Adenosine uptake was not dependent on extracellular K+ and was not affected by valinomycin. Although, K+-channel openers (BRL 34195 and nicorandil) as well as the K+-channel antagonist, glyburide, inhibited adenosine uptake, the inhibitory effect of BRL 34915 was not antagonized by glyburide. Rotenone and 2,4-dinitrophenol also inhibited adenosine uptake. Ionic dependence and metabolic energy dependence of adenosine uptake suggest that uptake is primarily an active process.
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页码:1 / 8
页数:8
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