ACID AND NEUTRAL INVERTASES IN THE MESOCARP OF DEVELOPING MUSKMELON (CUCUMIS-MELO L CV PRINCE) FRUIT

被引:41
作者
RANWALA, AP [1 ]
IWANAMI, SS [1 ]
MASUDA, H [1 ]
机构
[1] OBIHIRO UNIV AGR & VET MED,DEPT AGR CHEM,OBIHIRO,HOKKAIDO 080,JAPAN
关键词
D O I
10.1104/pp.96.3.881
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Acid and neutral invertases were found in the mesocarp of developing muskmelon (Cucumis melo L. cv Prince) fruit and the activities of these enzymes declined with maturation of the fruit, concomitantly with the accumulation of sucrose. Neutral invertase was only present in the soluble fraction and acid invertase was present in both the soluble and cell-wall fractions. The cell-wall fraction contained three types of acid invertase: a NaCl-released invertase; an EDTA-released invertase, and a tightly bound invertase that still remained on the cell wall after treatment with NaCl and EDTA. The soluble acid and neutral invertases could be separated from one another by chromatography on DEAE-cellulose and they exhibited clear differences in their properties, namely, in their pH optima, substrate specificity, K(m) values for sucrose, and inhibition by metal ions. The EDTA-released invertase and the soluble acid invertase were similar with regard to their chromatographic behavior on DEAE-cellulose, but the NaCl-released invertase was different because it was adsorbed to a column of CM-cellulose. The soluble acid invertase and two cell-wall bound invertases had very similar characteristics with regard to optimal pH and temperature, K(m) value for sucrose, and substrate specificity.
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页码:881 / 886
页数:6
相关论文
共 19 条
[1]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2]   PROPERTIES OF INVERTASES OF CULTURED SYCAMORE CELLS AND CHANGES IN THEIR ACTIVITY DURING CULTURE GROWTH [J].
COPPING, LG ;
STREET, HE .
PHYSIOLOGIA PLANTARUM, 1972, 26 (03) :346-+
[3]  
DEY PM, 1984, ADV ENZYMOL RAMB, V56, P141
[4]   SUCROSE PHOSPHATE SYNTHASE AND ACID INVERTASE AS DETERMINANTS OF SUCROSE CONCENTRATION IN DEVELOPING MUSKMELON (CUCUMIS-MELO L) FRUITS [J].
HUBBARD, NL ;
HUBER, SC ;
PHARR, DM .
PLANT PHYSIOLOGY, 1989, 91 (04) :1527-1534
[5]   PURIFICATION AND CHARACTERIZATION OF SOLUBLE (CYTOSOLIC) AND BOUND (CELL-WALL) ISOFORMS OF INVERTASES IN BARLEY (HORDEUM-VULGARE) ELONGATING STEM TISSUE [J].
KARUPPIAH, N ;
VADLAMUDI, B ;
KAUFMAN, PB .
PLANT PHYSIOLOGY, 1989, 91 (03) :993-998
[6]   PROPERTIES OF INVERTASES IN SUGAR STORAGE TISSUES OF CITRUS-FRUIT AND CHANGES IN THEIR ACTIVITIES DURING MATURATION [J].
KATO, T ;
KUBOTA, S .
PHYSIOLOGIA PLANTARUM, 1978, 42 (01) :67-72
[7]   WHEAT INVERTASES - CHARACTERIZATION OF CELL WALL-BOUND AND SOLUBLE FORMS [J].
KRISHNAN, HB ;
BLANCHETTE, JT ;
OKITA, TW .
PLANT PHYSIOLOGY, 1985, 78 (02) :241-245
[8]  
LESTER GE, 1985, SCI HORTIC-AMSTERDAM, V26, P323, DOI 10.1016/0304-4238(85)90016-0
[9]   SUCROSE METABOLISM IN NETTED MUSKMELON FRUIT DURING DEVELOPMENT [J].
LINGLE, SE ;
DUNLAP, JR .
PLANT PHYSIOLOGY, 1987, 84 (02) :386-389
[10]  
MASUDA H, 1987, AGR BIOL CHEM TOKYO, V51, P2309