ATTEMPTS TO IMPROVE THE SENSITIVITY OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR 2-METHYLISOBORNEOL

被引:5
作者
CHUNG, SY
机构
关键词
2-METHYLISOBORNEOL; ELISA; ANTIBODY; CAMPHOR CONJUGATE; ETHYLENEDIAMINE;
D O I
10.2166/wst.1992.0039
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
An enzyme-linked immunosorbent assay (ELISA) for 2-methylisoborneol (MIB) was described previously with a sensitivity of 1-mu-g/ml (Chung et al., 1990). In an attempt to increase the sensitivity, the following approaches were used: (1) new high-titer antibodies raised against a camphorethylenediamine-BSA conjugate; (2) use of a camphor-alkaline phosphatase conjugate as the detecting system; and (3) modification of the ELISA conditions by different blocking agents, microtiter plates, and plate shaking. Despite these approaches, the sensitivity of the ELISA remained at 1-mu-g/ml, indicating that the sensitivity most probably related to the antibody affinity for MIB and not to the assay conditions and antibody titer.
引用
收藏
页码:89 / 95
页数:7
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