Regulation of Amphiregulin mRNA by TGF-beta in the Human Lung Adenocarcinoma Cell Line A549

被引：18

作者：

Bennett, Kelly L. ^{[1
]}

Plowman, Gregory D. ^{[1
]}

Buckley, Sharon D. ^{[1
]}

Skonier, John ^{[1
]}

Purchio, A. F. ^{[1
]}

机构：

[1] Bristol Myers Squibb, Seattle, WA 98121 USA

来源：

GROWTH FACTORS | 1992年 / 7卷 / 03期

关键词：

TCF-beta; AR; EGF; growth inhibition;

D O I：

10.3109/08977199209046925

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Transforming growth factor beta is a strong growth inhibitor for many types of normal and transformed cells, although little is known on the mechanism of this anti-proliferative effect. The human lung adenocarcinoma cell line A549 is growth arrested by TGF-beta 1 and serves as a model for studying this effect. We describe that, concurrent with the inhibition of A549 cell growth, TGF-beta 1 treatment causes a dramatic reduction in the level of expression of the amphiregulin (AR) gene, a recently identified member of the EGF/TGF alpha family. Similar results were also observed with TGF-beta 2. Peak inhibition occurred at 24 hr of treatment and was reversible upon removal of TGF-beta 1. The level of AR protein secreted by A549 cells was also decreased by TGF-beta 1. In contrast, TGF-alpha mRNA was not detected in these cells regardless of TGF-beta 1 treatment. Another TGF-beta inhibited cell line, PC-3 (human prostatic adenocarcinoma) also exhibited a decrease in AR message levels following exposure to TGF-beta 1. The growth inhibitory effects of TGF-beta may in part be mediated by modulation of AR expression.

引用

页码：207 / 213

页数：8

共 50 条

[21] KIM SJ, 1989, J BIOL CHEM, V264, P7041
[22] KONDAIAH P, 1990, J BIOL CHEM, V265, P1089
[23] RNA MOLECULAR-WEIGHT DETERMINATIONS BY GEL-ELECTROPHORESIS UNDER DENATURING CONDITIONS, A CRITICAL RE-EXAMINATION
LEHRACH, H
DIAMOND, D
WOZNEY, JM
BOEDTKER, H
[J]. BIOCHEMISTRY, 1977, 16 (21) : 4743 - 4751
[24] INDUCTION OF C-SIS MESSENGER-RNA AND ACTIVITY SIMILAR TO PLATELET-DERIVED GROWTH-FACTOR BY TRANSFORMING GROWTH-FACTOR-BETA - A PROPOSED MODEL FOR INDIRECT MITOGENESIS INVOLVING AUTOCRINE ACTIVITY
LEOF, EB
PROPER, JA
GOUSTIN, AS
SHIPLEY, GD
DICORLETO, PE
MOSES, HL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (08) : 2453 - 2457
[25] LI L, 1990, J BIOL CHEM, V265, P1556
[26] ISOLATION AND PARTIAL CHARACTERIZATION OF A MR 32,000 PROTEIN WITH INHIBIN ACTIVITY FROM PORCINE FOLLICULAR-FLUID
LING, N
YING, SY
UENO, N
ESCH, F
DENOROY, L
GUILLEMIN, R
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (21) : 7217 - 7221
[27] EXPRESSION AND CHARACTERIZATION OF RECOMBINANT TGF-BETA-2 PROTEINS PRODUCED IN MAMMALIAN-CELLS
MADISEN, L
FARRAND, AL
LIOUBIN, MN
MARZOWSKI, J
KNOX, LB
WEBB, NR
LIM, J
PURCHIO, AF
[J]. DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1989, 8 (03): : 205 - 212
[28] MARQUARDT H, 1987, J BIOL CHEM, V262, P12127
[29] TYPE BETA-TRANSFORMING GROWTH-FACTOR IS AN INHIBITOR OF MYOGENIC DIFFERENTIATION
MASSAGUE, J
CHEIFETZ, S
ENDO, T
NADALGINARD, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) : 8206 - 8210
[30] THE TRANSFORMING GROWTH-FACTOR-BETA FAMILY
MASSAGUE, J
[J]. ANNUAL REVIEW OF CELL BIOLOGY, 1990, 6 : 597 - 641

← 1 2 3 4 5 →