NUCLEOTIDE-SEQUENCE ANALYSIS OF 95 KB NEAR THE 3' END OF THE MURINE T-CELL RECEPTOR-ALPHA-DELTA CHAIN LOCUS - STRATEGY AND METHODOLOGY

被引：46

作者：

WILSON, RK ^{[1
]}

KOOP, BF ^{[1
]}

CHEN, C ^{[1
]}

HALLORAN, N ^{[1
]}

SCIAMMIS, R ^{[1
]}

HOOD, L ^{[1
]}

机构：

[1] CALTECH, DIV BIOL, PASADENA, CA 91125 USA

来源：

GENOMICS | 1992年 / 13卷 / 04期

关键词：

D O I：

10.1016/0888-7543(92)90038-T

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nucleotide sequence of a region at the 3′ terminus of the murine T-cell receptor α δ chain locus is presented. This region, which encodes the constant region genes for α and δ chain polypeptides and all 50 joining gene segments for the α chain polypeptide, spans 94,647 bp and includes more than 50 noncoding sequence elements important for T-cell receptor gene rearrangement and expression. DNA sequencing of this region included complete analysis of two cosmid clones and five additional restriction fragments using a random subcloning approach with various manual and automated sequencing strategies. The automated sequencing strategies hold considerable promise for future large-scale DNA sequencing efforts. © 1992.

引用

页码：1198 / 1208

页数：11

共 59 条

[1] SHOTGUN DNA SEQUENCING USING CLONED DNASE I-GENERATED FRAGMENTS [J].