NUCLEOTIDE-SEQUENCE ANALYSIS OF 95 KB NEAR THE 3' END OF THE MURINE T-CELL RECEPTOR-ALPHA-DELTA CHAIN LOCUS - STRATEGY AND METHODOLOGY

被引:46
作者
WILSON, RK [1 ]
KOOP, BF [1 ]
CHEN, C [1 ]
HALLORAN, N [1 ]
SCIAMMIS, R [1 ]
HOOD, L [1 ]
机构
[1] CALTECH, DIV BIOL, PASADENA, CA 91125 USA
关键词
D O I
10.1016/0888-7543(92)90038-T
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The nucleotide sequence of a region at the 3′ terminus of the murine T-cell receptor α δ chain locus is presented. This region, which encodes the constant region genes for α and δ chain polypeptides and all 50 joining gene segments for the α chain polypeptide, spans 94,647 bp and includes more than 50 noncoding sequence elements important for T-cell receptor gene rearrangement and expression. DNA sequencing of this region included complete analysis of two cosmid clones and five additional restriction fragments using a random subcloning approach with various manual and automated sequencing strategies. The automated sequencing strategies hold considerable promise for future large-scale DNA sequencing efforts. © 1992.
引用
收藏
页码:1198 / 1208
页数:11
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