METHODS AND REAGENTS - PURIFICATION OF HIS-TAG FUSION PROTEINS FROM ESCHERICHIA-COLI

被引：97

作者：

HENGEN, PN

机构：

[1] National Cancer Institute, Frederick Cancer Research and Development Center, Frederick

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 1995年 / 20卷 / 07期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0968-0004(00)89045-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a contaminating protein from Escherichia coli found when histidine-tagged fusion proteins are purified by metal chelate affinity chromatography. For details on how to partake in the newsgroup, see the accompanying box.

引用

页码：285 / 286

页数：2

共 7 条

[1]

BAIER G, 1994, BIOTECHNIQUES, V17, P94

[2] EXPRESSION VECTORS FOR AFFINITY PURIFICATION AND RADIOLABELING OF PROTEINS USING ESCHERICHIA-COLI AS HOST [J].