THE EMBRYONIC EXPRESSION PATTERNS OF ZFH-1 AND ZFH-2, 2 DROSOPHILA GENES ENCODING NOVEL ZINC-FINGER HOMEODOMAIN PROTEINS

被引:164
作者
LAI, ZC
FORTINI, ME
RUBIN, GM
机构
[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720
[2] UNIV CALIF BERKELEY,HOWARD HUGHES MED INST,BERKELEY,CA 94720
关键词
TRANSCRIPTION FACTOR; EMBRYONIC DEVELOPMENT; NEUROGENESIS; MESODERM DIFFERENTIATION; ECTOPIC EXPRESSION; DROSOPHILA-MELANOGASTER;
D O I
10.1016/0925-4773(91)90049-C
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The zfh-1 and zfh-2 genes of D. melanogaster encode novel proteins containing both homeodomain and C2-H-2 zinc-finger DNA-binding motifs. Antisera against these proteins were used to investigate their expression patterns during embryonic development. The zfh-1 gene is expressed in the mesoderm of early embryos and in a number of mesodermally-derived structures of late embryos, including the dorsal vessel, support cells of the gonads, and segment-specific arrays of adult muscle precursors. In addition, zfh-1 is expressed in the majority of identified motor neurons of the developing CNS. The mesodermal zfh-1 expression requires the products of the twist and snail genes. The zfh-2 gene displays a more limited expression pattern, largely restricted to the CNS of late embryos. Ubiquitous zfh-1 expression in transgenic flies bearing an hsp70-zfh-1 construct has specific developmental consequences, including embryonic CNS defects as well as adult eye and bristle abnormalities. The expression patterns of zfh-1 and zfh-2 suggest that both genes may be involved in Drosophila neurogenesis and that zfh-1 may have additional functions in mesoderm development.
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页码:123 / 134
页数:12
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