POSTTRANSCRIPTIONAL SILENCING OF REPORTER TRANSGENES IN TOBACCO CORRELATES WITH DNA METHYLATION

被引：222

作者：

INGELBRECHT, I ^{[1
]}

VANHOUDT, H ^{[1
]}

VANMONTAGU, M ^{[1
]}

DEPICKER, A ^{[1
]}

机构：

[1] STATE UNIV GHENT,GENET LAB,B-9000 GHENT,BELGIUM

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 22期

关键词：

COSUPPRESSION; CYTOSINE METHYLATION; EPIGENETICS; NICOTIANA TABACUM; TRANSGENIC PLANT;

D O I：

10.1073/pnas.91.22.10502

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features-i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical moths CG and CXG.

引用

页码：10502 / 10506

页数：5

共 51 条

[1] EPIGENETIC REPEAT-INDUCED GENE SILENCING (RIGS) IN ARABIDOPSIS
ASSAAD, FF
TUCKER, KL
SIGNER, ER
[J]. PLANT MOLECULAR BIOLOGY, 1993, 22 (06) : 1067 - 1085
[2] Ausubel FM, 1987, CURRENT PROTOCOLS MO
[3] METHYLATION INDUCED PREMEIOTICALLY IN ASCOBOLUS - COEXTENSION WITH DNA REPEAT LENGTHS AND EFFECT ON TRANSCRIPT ELONGATION
BARRY, C
FAUGERON, G
ROSSIGNOL, JL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (10) : 4557 - 4561
[4] GENOMIC FOOTPRINTING REVEALS CELL TYPE SPECIFIC DNA-BINDING OF UBIQUITOUS FACTORS
BECKER, PB
RUPPERT, S
SCHUTZ, G
[J]. CELL, 1987, 51 (03) : 435 - 443
[5] BOLLMANN J, 1991, PLANT CELL, V3, P1327, DOI 10.1105/tpc.3.12.1327
[6] DNA METHYLATION INHIBITS TRANSCRIPTION INDIRECTLY VIA A METHYL-CPG BINDING-PROTEIN
BOYES, J
BIRD, A
[J]. CELL, 1991, 64 (06) : 1123 - 1134
[7] REPRESSION OF GENES BY DNA METHYLATION DEPENDS ON CPG DENSITY AND PROMOTER STRENGTH - EVIDENCE FOR INVOLVEMENT OF A METHYL-CPG BINDING-PROTEIN
BOYES, J
BIRD, A
[J]. EMBO JOURNAL, 1992, 11 (01) : 327 - 333
[8] BRUSSLAN JA, 1993, PLANT CELL, V5, P667, DOI 10.1105/tpc.5.6.667
[9] DEBLAERE R, 1985, NUCLEIC ACIDS RES, V13, P4777, DOI 10.1093/nar/13.13.4777
[10] ENGINEERING HERBICIDE RESISTANCE IN PLANTS BY EXPRESSION OF A DETOXIFYING ENZYME
DEBLOCK, M
BOTTERMAN, J
VANDEWIELE, M
DOCKX, J
THOEN, C
GOSSELE, V
MOVVA, NR
THOMPSON, C
VANMONTAGU, M
LEEMANS, J
[J]. EMBO JOURNAL, 1987, 6 (09) : 2513 - 2518

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