POSTTRANSCRIPTIONAL SILENCING OF REPORTER TRANSGENES IN TOBACCO CORRELATES WITH DNA METHYLATION

被引：222

作者：

INGELBRECHT, I ^{[1
]}

VANHOUDT, H ^{[1
]}

VANMONTAGU, M ^{[1
]}

DEPICKER, A ^{[1
]}

机构：

[1] STATE UNIV GHENT,GENET LAB,B-9000 GHENT,BELGIUM

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 22期

关键词：

COSUPPRESSION; CYTOSINE METHYLATION; EPIGENETICS; NICOTIANA TABACUM; TRANSGENIC PLANT;

D O I：

10.1073/pnas.91.22.10502

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Endogenous plant genes or transgenes can be silenced on introduction of homologous gene sequences. Here we document a reporter gene-silencing event in Nicotiana tabacum that has a distinctive combination of features-i.e., (i) silencing occurs by a posttranscriptional process, (ii) silencing correlates with DNA methylation, and (iii) this de novo methylation is not restricted to cytosines located in the symmetrical moths CG and CXG.

引用

页码：10502 / 10506

页数：5

共 51 条

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