GENETIC-ANALYSIS OF THE IMMUNITY REGION OF PHAGE-PLASMID-P4

被引：48

作者：

GHISOTTI, D ^{[1
]}

CHIARAMONTE, R ^{[1
]}

FORTI, F ^{[1
]}

ZANGROSSI, S ^{[1
]}

SIRONI, G ^{[1
]}

DEHO, G ^{[1
]}

机构：

[1] UNIV MILAN,DIPARTIMENTO GENET & BIOL MICRORGANISMI,VIA CELORIA 26,I-20133 MILAN,ITALY

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 22期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb02208.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the prophage P4, expression of the early genes is prevented by premature termination of transcription from the constitutive promoter P(LE). In order to identify the region coding for the immunity determinant, we cloned several fragments of P4 DNA and tested their ability to confer immunity to P4 superinfection. A 357 bp long fragment (P4 8418-8774) is sufficient to confer immunity to an infecting P4 phage and to complement the immunity-defective P4 cl405 mutant, both in the presence and in the absence of the helper phage P2. The immunity region covers P(LE) and the cl locus. We were unable to obtain evidence of translation of the region, thus we suggest that P4 immunity is not elicited by a protein but by a transcript (or transcripts) encoded by the region downstream of the promoter P(LE). The promoter P(LE) appears to be necessary for the expression of P4 immunity: fragments in which the P(LE) region is deleted did not complement P4 cl405 for lysogenization, although they still interfered with P4 growth. Two complementary sequences downstream of P(LE) (seqA and seqB) at the 5' and 3' ends of the immunity region play an essential role in the control of P4 immunity.

引用

页码：3405 / 3413

页数：9

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