A COMPARATIVE-STUDY OF PH/ACTIVITY PROFILES FOR THE ANAEROBIC H2O2 AND ALKYL HYDROPEROXIDE SUPPORTED N DEMETHYLATION OF N METHYLANILINE CATALYZED BY ALKALINE HEMATIN AND MICROSOMAL CYTOCHROME-P-450

被引：4

作者：

ADAMS, C ^{[1
]}

ADAMS, PA ^{[1
]}

机构：

[1] UNIV CAPE TOWN,SCH MED,DEPT CHEM PATHOL,BIOMEMBRANE RES UNIT,OBSERV 7925,CAPE TOWN,SOUTH AFRICA

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 1992年 / 45卷 / 01期

关键词：

D O I：

10.1016/0162-0134(92)84040-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A comparative study of the effect of pH on haemin-mediated H2O2 and alkyl hydroperoxide-supported N-demethylation of N-methylaniline and the corresponding cytochrome P-450 mediated process has been carried out. This extends previous studies and provides quantitative evidence that the same active oxygen species are involved in both the enzymic and metalloporphyrin mediated processes. Furthermore, this evidence clearly indicates that the environment at the point of catalysis in the enzyme active site differs considerably from that of the bulk solution.

引用

页码：47 / 52

页数：6

共 15 条

[1]

ADAMS C, 1986, S AFR J SCI, V82, P113

[2] FERRIPROTOPORPHYRIN-IX OXYGEN ACTIVATION INSERTION REACTIONS - THE NATURE OF THE INTERACTION BETWEEN FERRIPROTOPORPHYRIN-IX AND ANILINE (SUBSTRATE) [J].