1. 1. The amylase activities of serum, liver, pancreas, submaxillary and parotid glands, saliva, duodenum, colon, lung, heart, spleen, kidney and skeletal muscle of the domestic cat, were determined. 2. 2. As in most mammals, the highest amylase content was found in the pancreas. Levels in saliva and salivary glands were very low. 3. 3. Amylase activities of the serum, liver and pancreas have an optimum pH 7.0-7.5. 4. 4. Amylases of serum, liver and pancreas were inactivated by removal of chloride and these activities mainly restored by addition of chloride. © 1979.