CHARACTERIZATION OF THE PROMOTER REGION OF THE BASIDIOMYCETE LENTINUS-EDODES LE.RAS GENE

被引：14

作者：

KAJIWARA, S ^{[1
]}

SHISHIDO, K ^{[1
]}

机构：

[1] TOKYO INST TECHNOL, DEPT LIFE SCI, MIDORI KU, YOKOHAMA, KANAGAWA 227, JAPAN

来源：

FEMS MICROBIOLOGY LETTERS | 1992年 / 92卷 / 02期

关键词：

PROMOTER; TRANSCRIPTIONAL INITIATION SITE; GENE; RAS; LENTINUS-EDODES;

D O I：

10.1016/0378-1097(92)90503-G

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An analysis of the 2.7-kb nucleotidc sequence including the 5'-flanking region of the basidiomycete Lentinus edodes Le.ras-encoding gene revealed that the promoter region contains a CAAT box, a CACCC box, two consecutive TATAAA boxes, and a CT-rich sequence element, in that order, from upstream to downstream. One major and two alternative transcriptional initiation sites are located 132 nucleotides and 85 and 90 nucleotides downstream from the downstream TATAAA box, and the major site is positioned just in the CT-rich sequence.

引用

页码：147 / 150

页数：4

共 16 条

[11] FINE-STRUCTURE GENETIC-ANALYSIS OF A BETA-GLOBIN PROMOTER [J].

MYERS, RM ;

TILLY, K ;

MANIATIS, T .

SCIENCE, 1986, 232 (4750) :613-618

[12] TRANSCRIPTIONAL CONTROL OF THE HUMAN HARVEY RAS PROTOONCOGENE - ROLE OF MULTIPLE ELEMENTS IN THE PROMOTER REGION [J].

NAGASE, T ;

UENO, Y ;

ISHII, S .

GENE, 1990, 94 (02) :249-253

[13]

Sambrook J., 1989, MOL CLONING LAB MANU

[14] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[15]

YAMAMOTO F, 1988, ONCOGENE RES, V3, P125

[16] IMPROVED M13 PHAGE CLONING VECTORS AND HOST STRAINS - NUCLEOTIDE-SEQUENCES OF THE M13MP18 AND PUC19 VECTORS [J].

YANISCHPERRON, C ;

VIEIRA, J ;

MESSING, J .

GENE, 1985, 33 (01) :103-119

← 1 2 →