THE ROLE OF RAB3A IN NEUROTRANSMITTER RELEASE

被引:419
作者
GEPPERT, M
BOLSHAKOV, VY
SIEGELBAUM, SA
TAKEI, K
DECAMILLI, P
HAMMER, RE
SUDHOF, TC
机构
[1] UNIV TEXAS, SW MED CTR, DEPT MOLEC GENET, DALLAS, TX 75235 USA
[2] UNIV TEXAS, SW MED CTR, DEPT BIOCHEM, DALLAS, TX 75235 USA
[3] UNIV TEXAS, SW MED CTR, HOWARD HUGHES MED INST, DALLAS, TX 75235 USA
[4] COLUMBIA UNIV, HOWARD HUGHES MED INST, CTR NEUROBIOL & BEHAV, DEPT PHARMACOL, NEW YORK, NY 10032 USA
[5] YALE UNIV, SCH MED, DEPT CELL BIOL, NEW HAVEN, CT 06510 USA
[6] YALE UNIV, SCH MED, HOWARD HUGHES MED INST, NEW HAVEN, CT 06510 USA
关键词
D O I
10.1038/369493a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
THE small GTP-binding protein Rab3A is a Rab family member(1-3) that is abundant in brain synaptic vesicles(4,5). Here we show that mice in which the rab3A gene has been mutated by homologous recombination do not express Rab3A but are viable and fertile. Electrophysiological recordings in hippocampal CA1 pyramidal cells indicate that most of their synaptic parameters are also normal, although synaptic depression after short trains of repetitive stimuli (15-30 stimuli at 14 Hz) is significantly increased. Levels of the Rab3A-binding protein rabphilin are decreased by 70%, but expression of more than 20 other synaptic proteins is unchanged. No compensatory changes were detected in other GTP-binding proteins or in proteins that interact with Rab3. Rab3A thus appears not to be essential for synaptic vesicle exocytosis but to play a role in the recruitment of synaptic vesicles for exocytosis during repetitive stimulation.
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页码:493 / 497
页数:5
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