STRUCTURE OF THE MOUSE BASIGIN GENE, A UNIQUE MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY

Basigin is a membrane glycoprotein belonging to the immunoglobulin superfamily, The mouse basigin gene was isolated from a genomic DNA library of the BALB/c mouse, and the structure of the gene and its flanking region (11.8 kb) was completely determined, The mouse basigin gene consists of seven exons and six introns spanning 7.5 kb, The distance between the first and second exons is 5.1 kb, The first immunoglobulin-like domain of the basigin molecule is encoded by the second and third exons, and the second immunoglobulin-like domain by the fourth and fifth exons, The fifth exon encodes not only the C proximal portion of the second immunoglobulin-like domain, but also the transmembrane domain and a small portion of the cytoplasmic domain, Thus, the organization of the basigin gene is unique, The 5' upstream sequence of the basigin gene contains no TATA box or CAAT box, but has a CpG-rich island, The BALB/c genomic sequence of all seven exons is consistent with the cDNA sequences of the 129/SV and Swiss mice except several minor substitutions in the 8'-terminal sequence of the 3'-noncoding region, No protein polymorphism has so far been found in basigin of different mouse strains.