PREPARATION AND PROPERTIES OF SOLUBLE INSOLUBLE IMMOBILIZED PROTEASES

被引：63

作者：

FUJIMURA, M

MORI, T

TOSA, T

机构：

[1] Tanabe Seiyaku Co, Osaka, Jpn, Tanabe Seiyaku Co, Osaka, Jpn

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1987年 / 29卷 / 06期

关键词：

BIOCHEMICAL ENGINEERING - COPOLYMERS - Applications - POLYMETHYL METHACRYLATE - Applications;

D O I：

10.1002/bit.260290612

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In order to carry out an effective enzyme reaction, the preparation of soluble-insoluble immobilized enzyme was investigated. Proteases were selected as model enzymes, and their immobilization was carried out by using an enteric coating polymer as a carrier. Among the polymers tested, methacrylic acid-methacrylate-metal methacrylate copolymer (MPM-06) gave the most active soluble-insoluble immobilized papain. This immobilized papain showed insoluble form below pH 4. 8 and soluble form above pH 5. 8; it was also soluble in water-miscible organic solvent. It was reusable and more stable with heat and water miscible organic solvents than native proteases. Furthermore various proteases could be immobilized by using MPM-06 with high activity. Chymotrypsin immobilized by this method catalyzed the effective peptide synthesis in a heterogeneous reaction system containing water-miscible organic solvent.

引用

页码：747 / 752

页数：6