CONSTRUCTION AND EXPRESSION OF A METALLOTHIONEIN BETA-GLUCURONIDASE GENE FUSION

被引：23

作者：

HATTORI, J ^{[1
]}

LABBE, H ^{[1
]}

MIKI, BL ^{[1
]}

机构：

[1] AGR CANADA,PLANT RES CTR,OTTAWA K1A 0C6,ON,CANADA

来源：

GENOME | 1994年 / 37卷 / 03期

关键词：

METALLOTHIONEIN-BETA-GLUCURONIDASE FUSION; CADMIUM; TRANSGENIC PLANTS;

D O I：

10.1139/g94-071

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A gene fusion consisting of the Chinese hamster metallothionein II and beta-glucuronidase coding regions was constructed. The fusion protein expressed in Escherichia coli retained cadmium-binding capacity and beta-glucuronidase activity. When expressed from the constitutive 35S promoter in transgenic tobacco, the levels of Cd-109 accumulation in leaves were reduced to about 70% of those untransformed control plants. Metallothionin-beta-glucuronidase did not sequester a significant proportion of the leaf Cd-109 taken up through the roots in vitro and therefore a sink for Cd was not created.

引用

页码：508 / 512

页数：5

共 34 条

[11] PHYTOCHELATINS, A CLASS OF HEAVY-METAL-BINDING PEPTIDES FROM PLANTS, ARE FUNCTIONALLY ANALOGOUS TO METALLOTHIONEINS [J].