CHIRAL SEPARATION OF DRUGS BY CAPILLARY ELECTROPHORESIS USING BETA-CYCLODEXTRIN POLYMER

The direct separation of enantiomers of trimetoquinol hydrochloride, related substances and some other drugs was investigated by capillary electrophoresis employing six kinds of cyclodextrins (CDs). Enantiomeric recognition of trimetoquinol and related substances was successfully achieved by using beta-cyclodextrin (beta-CD), heptakis(2,6- di-O-methyl)-beta-cyclodextrin (DM-beta-CD) and beta-CD polymer. beta-CD polymer was especially effective for the separation of both enantiomers and different solutes. The effects of the amount of beta-CD polymer added to the background electrolyte, the pH of the buffer solution and some additives such as an organic solvent or a surfactant on the resolution of enantiomers were examined. The best enantioseparation was obtained by employing 5-7% beta-CD polymer in an acidic solution with a surfactant.