CLONING AND EXPRESSION OF THE ASPARTATE CARBAMOYLTRANSFERASE GENE FROM TREPONEMA-DENTICOLA

被引：11

作者：

ISHIHARA, K

ISHIHARA, M

TAKAZOE, I

OKUDA, K

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 10期

关键词：

D O I：

10.1128/AEM.58.10.3399-3403.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Treponema denticola seems to play a central role in the etiology of human periodontal disease. We have cloned an antigenic protein-coding sequence from T. denticola ATCC 33520. The protein-coding region was found to be a 3-kbp HindIII-HindIII fragment. The open reading frame consists of 1,426 bp and codes for a protein with an M(r) of 54,919. The deduced amino acid sequence showed 33.8% homology with that of the aspartate carbamoyltransferase of Escherichia coli. The gene products showed aspartate carbamoyltransferase activity.

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页码：3399 / 3403

页数：5

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