DEVELOPMENT, IDENTIFICATION, AND CHARACTERIZATION OF A GLYPHOSATE-TOLERANT SOYBEAN LINE

被引：414

作者：

PADGETTE, SR ^{[1
]}

KOLACZ, KH ^{[1
]}

DELANNAY, X ^{[1
]}

RE, DB ^{[1
]}

LAVALLEE, BJ ^{[1
]}

TINIUS, CN ^{[1
]}

RHODES, WK ^{[1
]}

OTERO, YI ^{[1
]}

BARRY, GF ^{[1
]}

EICHHOLTZ, DA ^{[1
]}

PESCHKE, VM ^{[1
]}

NIDA, DL ^{[1
]}

TAYLOR, NB ^{[1
]}

KISHORE, GM ^{[1
]}

机构：

[1] ASGROW SEED CO, KALAMAZOO, MI 49002 USA

来源：

CROP SCIENCE | 1995年 / 35卷 / 05期

关键词：

D O I：

10.2135/cropsci1995.0011183X003500050032x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Glyphosate (N-phosphonomethyl-glycine) is the active ingredient in the nonselective herbicide Roundup. The sensitivity of crop plants to glyphosate has limited its in-season use as a postemergence herbicide. The extension of the use of Roundup herbicide to allow in-season application in major crops such as soybeans [Glycine mar (L.) Merr.] would provide nerv weed control options for farmers. A glyphosate-tolerant soybean line, 40-3-2, was obtained through expression of the bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EPSPS) enzyme from Agrobacterium sp. strain CP4. Line 40-3-2 is highly tolerant to glyphosate, showing no visual injury after application of up to 1.68 kg acid equivalent (a.e.) ha(-1) of glyphosate under field conditions. Molecular characterization studies determined that the single genetic insert in fine 40-3-2 contains only a portion of the cauliflower mosaic virus 35S promoter (P-E35S), the Petunia hybrida EPSPS chloroplast transit peptide (CTP), the CP4 EPSPS gene, and a portion of the 3' nontranslated region of the nopaline synthase gene (NOS 3') terminator. Inheritance studies have shown that the transgene behaves as a single dominant gene and is stable over several generations.

引用

页码：1451 / 1461

页数：11

共 48 条

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