LARGE-SCALE SCREENING FOR HUMAN PARVOVIRUS B19 DNA IN CLINICAL SPECIMENS BY DOT-BLOT HYBRIDIZATION AND POLYMERASE CHAIN-REACTION

被引：13

作者：

YOTO, Y ^{[1
]}

KUDOH, T ^{[1
]}

HASEYAMA, K ^{[1
]}

SUZUKI, N ^{[1
]}

MATSUNAGA, Y ^{[1
]}

CHIBA, S ^{[1
]}

机构：

[1] NATL INST HLTH, TOKYO 141, JAPAN

来源：

JOURNAL OF MEDICAL VIROLOGY | 1995年 / 47卷 / 04期

关键词：

HUMAN PARVOVIRUS B19; ERYTHEMA INFECTIOSUM; DOT BLOT HYBRIDIZATION; POLYMERASE CHAIN REACTION;

D O I：

10.1002/jmv.1890470424

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Large-scale screening for human parvovirus B19 (B19) DNA in serum samples was carried out by both dot blot hybridization and the polymerase chain reaction (PCR). Dot blot hybridization was undertaken with a digoxigenin-labeled DNA probe. Serum samples from four patients were pooled and tested by a dot blot hybridization assay. When a dot was positive, each of the four samples was tested separately to identify the positive sample. The PCR template was the DNA extracted from mixed serum samples from 10 patients. When B19 DNA was positive by PCR, each of the ten samples was tested separately. A total of 7,969 serum samples were tested by dot blot hybridization and 15 samples (11 patients) were positive for B19 DNA; 7,038 serum samples were tested by PCR and 71 samples (50 patients) were positive. Large-scale screening for B19 DNA by PCR suggested a broader spectrum of clinical manifestations associated with B19 infection. (C) 1995 Wiley-Liss, Inc.

引用

页码：438 / 441

页数：4

共 19 条

[1]

ANDERSON MJ, 1983, LANCET, V1, P1378

[2] DETECTION OF B19-PARVOVIRUS INFECTIONS BY A DOT BLOT HYBRIDIZATION ASSAY USING A DIGOXIGENIN-LABELED PROBE [J].