VANILLATE HYDROXYLASE FROM THE WHITE ROT BASIDIOMYCETE PHANEROCHAETE-CHRYSOSPORIUM

被引：47

作者：

YAJIMA, Y ^{[1
]}

ENOKI, A ^{[1
]}

MAYFIELD, MB ^{[1
]}

GOLD, MH ^{[1
]}

机构：

[1] OREGON GRAD CTR STUDY & RES,DEPT CHEM & BIOCHEM SCI,BEAVERTON,OR 97005

来源：

ARCHIVES OF MICROBIOLOGY | 1979年 / 123卷 / 03期

关键词：

Basidiomycete; Lignin biodegradation; Methoxy-p-hydroquinone; Monooxygenase; Phanerochaete chrysosporium; Vanillate hydroxylase; Vanillic acid;

D O I：

10.1007/BF00406669

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A soluble enzyme fraction from Phanerochaete chrysosporium catalyzed the oxidative decarboxylation of vanillic acid to methoxy-p-hydroquinone. The enzyme, partially purified by ammonium sulfate precipitation, required NADPH and molecular oxygen for activity. NADH was not effective. Optimal activity was displayed between pH 7.5-8.5. Neither EDTA, KCN, NaN3, nor o-phenanthroline (5 mM) were inhibitory. The enzyme was inducible with maximal activity displayed after incubation of previously grown cells with 0.1% vanillate for 30h. © 1979 Springer-Verlag.

引用

页码：319 / 321

页数：3