ANALYSIS OF PHOSPHOLIPIDS IN BRAIN-TISSUE BY P-31 NMR AT DIFFERENT COMPOSITIONS OF THE SOLVENT SYSTEM CHLOROFORM METHANOL WATER

被引：67

作者：

EDZES, HT

TEERLINK, T

VANDERKNAAP, MS

VALK, J

机构：

[1] FREE UNIV AMSTERDAM, FAC CHEM, DEPT PHYS & THEORET CHEM, 1007 MC AMSTERDAM, NETHERLANDS

[2] VRIJE UNIV AMSTERDAM HOSP, DEPT CLIN CHEM, AMSTERDAM, NETHERLANDS

[3] UNIV UTRECHT, CHILDRENS HOSP, WILHELMINA KINDERZIEKENHUIS, DEPT CHILD NEUROL, UTRECHT, NETHERLANDS

[4] VRIJE UNIV AMSTERDAM HOSP, DEPT DIAGNOST RADIOL, AMSTERDAM, NETHERLANDS

来源：

MAGNETIC RESONANCE IN MEDICINE | 1992年 / 26卷 / 01期

关键词：

D O I：

10.1002/mrm.1910260106

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Brain phospholipids can be quantitated by high‐resolution 31P NMR of crude brain tissue extracts in the solvent system chloroform‐methanol‐water (+EDTA) which was introduced recently (P. Meneses and T. Glonek, J. Lipid Res. 29, 679 (1988)). Phospholipid resonance positions depend on the type of tissue extract and on solvent composition. The effects of systematic variation of the solvent system on phospholipid NMR profiles are presented. Resolution can be optimized by adjustment of the solvent composition. Virtually all phospholipid classes can be resolved, and the major phospholipids in brain: sphingo‐myelin and phosphatidyl‐choline, ‐serine, ‐inositol, ‐ethanolamine, and ‐ethanolamine plasmalogen can be quantitated easily. Additional resonances have been assigned to phosphatidylcholine plasmalogen, alkylacyl‐phosphatidylcholine and phosphatidylinositolbis‐phosphate. NMR offers a rapid method for quantitative analysis of the phospholipid composition in brain tissue which requires minimum sample handling. Copyright © 1992 Wiley‐Liss, Inc., A Wiley Company

引用

页码：46 / 59

页数：14

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