SUBSTITUTION OF LYSINE FOR ARGININE IN THE N-TERMINAL 217TH AMINO-ACID RESIDUE OF THE H-GAMMA-II OF STAPHYLOCOCCAL GAMMA-HEMOLYSIN LOWERS THE ACTIVITY OF THE TOXIN

被引：8

作者：

SUDO, K ^{[1
]}

CHOORIT, W ^{[1
]}

ASAMI, I ^{[1
]}

KANEKO, J ^{[1
]}

MURAMOTO, K ^{[1
]}

KAMIO, Y ^{[1
]}

机构：

[1] TOHOKU UNIV, FAC AGR, DEPT APPL BIOL CHEM, AOBA KU, SENDAI, MIYAGI 981, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 09期

关键词：

D O I：

10.1271/bbb.59.1786

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The staphyloccocal toxin gamma-hemolysin consists of two protein components, LukF and H gamma II. Staphylococcus aureus P83 was found to have five components, LukF, LukF-PV, LukM, LukS, and H gamma II for leukocidin or gamma-hemolysin. H gamma II of S. aureus P83 was demonstrated to be a naturally-occurring analogous molecule of H gamma II [H gamma II(P83)], in which the 217th arginine residue was replaced by lysine. The H gamma II(P83) showed about 50% of the hemolytic activity of normal H gamma II in the presence of LukF.

引用

页码：1786 / 1789

页数：4

共 12 条

[1] EXISTENCE OF A NEW-PROTEIN COMPONENT WITH THE SAME FUNCTION AS THE LUKF COMPONENT OF LEUKOCIDIN OR GAMMA-HEMOLYSIN AND ITS GENE IN STAPHYLOCOCCUS-AUREUS P83 [J].

CHOORIT, W ;

KANEKO, J ;

MURAMOTO, K ;

KAMIO, Y .

FEBS LETTERS, 1995, 357 (03) :260-264

[2]

Chou P Y, 1978, Adv Enzymol Relat Areas Mol Biol, V47, P45

[3] THE GAMMA-HEMOLYSIN LOCUS OF STAPHYLOCOCCUS-AUREUS COMPRISES 3 LINKED GENES, 2 OF WHICH ARE IDENTICAL TO THE GENES FOR THE F AND S COMPONENTS OF LEUKOCIDIN [J].

COONEY, J ;

KIENLE, Z ;

FOSTER, TJ ;

OTOOLE, PW .

INFECTION AND IMMUNITY, 1993, 61 (02) :768-771

[4] ANALYSIS OF ACCURACY AND IMPLICATIONS OF SIMPLE METHODS FOR PREDICTING SECONDARY STRUCTURE OF GLOBULAR PROTEINS [J].

GARNIER, J ;

OSGUTHORPE, DJ ;

ROBSON, B .

JOURNAL OF MOLECULAR BIOLOGY, 1978, 120 (01) :97-120

[5] THE 2 STAPHYLOCOCCAL BICOMPONENT TOXINS, LEUKOCIDIN AND GAMMA-HEMOLYSIN, SHARE ONE-COMPONENT IN COMMON [J].

KAMIO, Y ;

RAHMAN, A ;

NARIYA, H ;

OZAWA, T ;

IZAKI, K .

FEBS LETTERS, 1993, 321 (01) :15-18

[6] LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMINO-ACIDS AFTER GAS-PHASE HYDROLYSIS AND DERIVATIZATION WITH (DIMETHYLAMINO)AZOBENZENESULFONYL CHLORIDE [J].

KNECHT, R ;

CHANG, JY .

ANALYTICAL CHEMISTRY, 1986, 58 (12) :2375-2379

[7] IMPROVED METHOD FOR PURIFICATION OF LEUKOCIDIN AND GAMMA-HEMOLYSIN COMPONENTS FROM STAPHYLOCOCCUS-AUREUS [J].

NARIYA, H ;

ASAMI, I ;

OZAWA, T ;

BEPPU, Y ;

IZAKI, K ;

KAMIO, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (12) :2198-2199

[8] INACTIVATION OF GAMMA-HEMOLYSIN H-GAMMA-II COMPONENT BY ADDITION OF MONOSIALOGANGLIOSIDE GM1 TO HUMAN ERYTHROCYTE [J].

OZAWA, T ;

KANEKO, J ;

NARIYA, H ;

IZAKI, K ;

KAMIO, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (03) :602-605

[9] NUCLEOTIDE-SEQUENCE OF LEUKOCIDIN S-COMPONENT GENE (LUKS) FROM METHICILLIN RESISTANT STAPHYLOCOCCUS-AUREUS [J].

RAHMAN, A ;

IZAKI, K ;

KATO, I ;

KAMIO, Y .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 181 (01) :138-144

[10] GAMMA-HEMOLYSIN GENES IN THE SAME FAMILY WITH LUKF AND LUKS GENES IN METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS [J].

RAHMAN, A ;

IZAKI, K ;

KAMIO, Y .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (07) :1234-1236

← 1 2 →