De novo protein design: what are we learning?

被引：70

作者：

DeGrado, William F. ^{[1
]}

Raleigh, Daniel P. ^{[1
]}

Handel, Tracey ^{[1
]}

机构：

[1] Du Pont Merck Pharmaceut Co, Du Pont Expt Stn, POB 80328, Wilmington, DE 19880 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1991年 / 1卷 / 06期

关键词：

D O I：

10.1016/0959-440X(91)90095-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The field of de novo design is examined in the light of current theories of protein folding. The most carefully characterized designed proteins show many of the characteristics of molten globules or protein folding intermediates. Thus, it is unclear whether or not the objective of designing proteins that fully mimic the native states of natural proteins has been achieved.

引用

页码：984 / 993

页数：10

共 63 条

[1] STRUCTURE OF CHICKEN MUSCLE TRIOSE PHOSPHATE ISOMERASE DETERMINED CRYSTALLOGRAPHICALLY AT 2.5A RESOLUTION USING AMINO-ACID SEQUENCE DATA [J].

BANNER, DW ;

BLOOMER, AC ;

PETSKO, GA ;

PHILLIPS, DC ;

POGSON, CI ;

WILSON, IA ;

CORRAN, PH ;

FURTH, AJ ;

MILMAN, JD ;

OFFORD, RE ;

PRIDDLE, JD ;

WALEY, SG .

NATURE, 1975, 255 (5510) :609-614

[2] CHARACTERIZATION OF A PARTLY FOLDED PROTEIN BY NMR METHODS - STUDIES ON THE MOLTEN GLOBULE STATE OF GUINEA-PIG ALPHA-LACTALBUMIN [J].

BAUM, J ;

DOBSON, CM ;

EVANS, PA ;

HANLEY, C .

BIOCHEMISTRY, 1989, 28 (01) :7-13

[3] SECONDARY STRUCTURE OF THE DESIGNED PEPTIDE ALPHA-1 DETERMINED BY NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY [J].

CIESLA, DJ ;

GILBERT, DE ;

FEIGON, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (10) :3957-3961

[4] ALPHA-HELICAL COILED COILS AND BUNDLES - HOW TO DESIGN AN ALPHA-HELICAL PROTEIN [J].

COHEN, C ;

PARRY, DAD .

PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1990, 7 (01) :1-15

[5]

CREIGHTON TE, 1990, BIOCHEM J, V270, P1

[6] THE DESIGN OF A 4-HELIX BUNDLE PROTEIN [J].

DEGRADO, WF ;

REGAN, L ;

HO, SP .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1987, 52 :521-526

[7] PROTEIN DESIGN, A MINIMALIST APPROACH [J].

DEGRADO, WF ;

WASSERMAN, ZR ;

LEAR, JD .

SCIENCE, 1989, 243 (4891) :622-628

[8] Characterization of protein folding intermediates [J].

Dobson, Christopher M. .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1991, 1 (01) :22-27

[9] ALPHA-LACTALBUMIN - COMPACT STATE WITH FLUCTUATING TERTIARY STRUCTURE [J].

DOLGIKH, DA ;

GILMANSHIN, RI ;

BRAZHNIKOV, EV ;

BYCHKOVA, VE ;

SEMISOTNOV, GV ;

VENYAMINOV, SY ;

PTITSYN, OB .

FEBS LETTERS, 1981, 136 (02) :311-315

[10] MONOOXYGENASE ACTIVITY OF HUMAN-HEMOGLOBIN - ROLE OF QUATERNARY STRUCTURE IN THE PREPONDERANT ACTIVITY OF THE BETA-SUBUNITS WITHIN THE TETRAMER [J].

FERRAIOLO, BL ;

ONADY, GM ;

MIEYAL, JJ .

BIOCHEMISTRY, 1984, 23 (23) :5528-5534

← 1 2 3 4 5 6 7 →