TRANSGENIC TOBACCO PLANTS WITH T-DNA PHYTOHORMONE SYNTHESIS GENES

被引：14

作者：

ONDREJ, M

BAVRINA, TV

DUDKO, N

HROUDA, M

KREKULE, J

LOZHNIKOVA, VN

MACHACKOVA, I

SEIDLOVA, F

VLASAK, J

机构：

[1] Institute of Plant Molecular Biology, Czechoslovak Academy of Sciences, České Budějovice, 370 05

[2] Institute of Plant Physiology of A. K. Timiryazev, Academy of Sciences of USSR, Moscow, 127276

[3] Research Institute for Crop Production, Praha 6, 16100

来源：

BIOLOGIA PLANTARUM | 1991年 / 33卷 / 01期

关键词：

D O I：

10.1007/BF02873786

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Agrobacterium tumefaciens binary vectors carrying kanamycin resistance gene and either C58-T-DNA gene 4 for cytokinin synthesis or genes 1 and 2 for auxin synthesis were constructed and used for transformation of a short-day tobacco Maryland Mammoth. Kanamycin resistant plants were regenerated from a small fraction of transformed tissue and the presence of T-DNA in their genome was verified by Southern blotting. The level of endogenous cytokinin in plants transgenic for gene 4 and the level of endogenous IAA in those transgenic for genes 1 and 2 increased by more than 100%. A number of morphological characteristics distinguish them from untransformed controls.

引用

页码：40 / +

页数：1